Isolation, screening and characterization of yeasts with amyloytic, lipolytic, and proteolytic activities from the surface of Philippine bananas (Musa spp.)

Issue Date

6-2014

Abstract

Philippine fruits are rarely exploited as microbiological habitats from where yeasts with potential economic benefits can be isolated and identified. In this study, one hundred seventy yeasts were isolated from 5 banana samples. Amylase, lipase, and protease activity were tested on starch agar, Sierra’s medium with tween 80 and casein agar, respectively. Yeasts that tested positive for amylase, lipase, and protease were numbered 29, 76, and 34, respectively. Phenotypic identification of 15 yeasts with moderate to high enzyme activities was done and seven representative strains of the identified species were further subjected to D1/D2 26S ribosomal RNA gene (D1/D2 26S rRNA) sequencing. The yeasts with amylolytic, lipolytic, and/or proteolytic activities were identified as Brandoniozyma complexa, Candida wangnamkhiaoensis, Debaryomyces nepalensis, Hypopichia burtonii, Kodamaea ohmerii, Pichia anomala, Pseudozyma hubeiensis, P. prolifica, and P. pruni.

Source or Periodical Title

Philippine Journal of Science

ISSN

0031-7683

Volume

143

Issue

1

Page

81-87

Document Type

Article

Physical Description

tables

Language

English

Subject

Bananas, Enzymes, Yeast

Digital Copy

yes

Link to Full Text

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