Toward the utilization of OMICS technology in Philippine entomological research: Optimization of transcriptome and metagenome sequencing in selected insect species.
Issue Date
10-2017
Abstract
Omics research has paved its way in the Philippines through the advent of next generation sequencing (NGS) technologies available at the Philippine Genome Center. This study aims to add strength to the omics technique adopted in the institute by facilitating studies on transcriptomics and metagenomics of organisms with less complex genomes such as insects. Conducting these techniques that are more feasible for the NGS platforms would then enhance utility. Insect transcriptomics and metagenomics enable the generation of molecular toolbox that can be used to understand various biological processes in insects. Organisms of interest include a parasitoid (Comperiella sp.) and stingless bees (Tetragonula sp.) for transcriptomics, and a termite (Microcerotermes sp.) for metagrnomics. Optimization of nucleic acid template preparation was done to ensure good quality output for high-throughput sequencing and bioinformatics.
Source or Periodical Title
The Philippine Entomologist
ISSN
0048-3753
Volume
31
Issue
2
Page
172-173
Document Type
Article
Frequency
semi-annually
Language
English
Recommended Citation
Macareñas-Bautista, Ma. Anita; Camposano, Jessa Mae T.; Ballesteros, Alfredo Jose C.; Cleofe, Maria Almira S.; Abesamis, Kim Ivan; Llames, Jo Hannah S.; Nahar, Shamdee N.; Sabban, Emilia; Mostoles, Maria Dulce J.; Navasero, Marcela M.; and Navasero, Mario V., "Toward the utilization of OMICS technology in Philippine entomological research: Optimization of transcriptome and metagenome sequencing in selected insect species." (2017). Journal Article. 4131.
https://www.ukdr.uplb.edu.ph/journal-articles/4131
En – AGROVOC descriptors
COMPERIELLA; MELIPONA; MICROCEROTERMES; PARASITOIDS; GENOMICS; GENOMES; EPIGENETICS; TRANSCRIPTOME; GENETIC RESOURCES; RNA SEQUENCE