Enhanced plantlet regeneration and in vitro root production in vetiver [Vetiveria zizanioides (L.) Nash]

Issue Date

12-2019

Abstract

In vitro plantlet regeneration and production of roots in the shoot base explants of vetiver [Vetiveria zizanioides (L.) Nash] was investigated using Murashige and Skoog’s (MS) medium supplemented with different types and concentrations of plant growth regulators. Extensive shoot proliferation was maintained at 5 mg L-1 6-benzylaminopurine (BAP). Increasing the concentration of BAP to 20 mg L-1 significantly inhibited the growth of shoots. Root production was greatly enhanced by the addition of either 0.25 mg L-1 naphthaleneacetic acid (NAA) or 20 mg L-1 indole-3-butyric acid (IBA). Roots induced by NAA were short and thick, whereas those induced by IBA were long, thin and fibrous. Supplementation of 0.5 mg L-1 NAA to MS basal medium and further enrichment with 0.5 mg L-1 kinetin (Ki) significantly enhanced the proliferation of roots. However, addition of 0.2 mg L-1 paclobutrazol (PBZ) into the NAA-supplemented MS medium suppressed both shoot and root growth of vetiver.

Source or Periodical Title

Philippine Agricultural Scientist, The (Formerly: The Philippine Agriculturist)

ISSN

0031-7454

Volume

95

Issue

4

Page

344-351

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

quarterly

Physical Description

illustrations; tables; references

Language

English

Subject

in vitro culture, plant regeneration, root production, shoot induction, Vetiveria zizanioides (L.) Nash

En – AGROVOC descriptors

VETIVERIA ZIZANIOIDES; SEEDLINGS; REGENERATION; ROOT PRODUCTIVITY; IN VITRO CULTURE; MICROPROPAGATION

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