Development of probiotic nutraceuticals using indigenous plants and lactic acid bacteria

Date

2016

Abstract

Leaf extracts of moringa, bitter gourd, avocado, aratiles and piper betle were used for selection of anti-diarrheal plant. Based on the clarity of zones of inhibitions, piper beetle leaf extract exhibited the highest antimicrobial activity against diarrhea-causing pathogens like Salmonella typhimurium, Eschirichia coli and E. coli 0157:H7. Among the sixty lactic acid bacteria isolated from Phiippine plants, ten isolates that survived better against 30% pure piper betle extract were selected for further tests. Par5, NN39 and Lk13 showed the highest antimicrobial properties against E.coli, B. cereus and S. aureus. All the ten selected LAB showed susceptibility to ampicillin concentration higher than 0.125uh/mL. But only Par5, NN39, SP5 and SP6 were resistant at 128 ug/mL streptomycin concentration. Although exposure on artificial gastric juice (pH2) after 3h resulted to very low survival, they showed good survival on simulated intestinal fluid with LK 13 having the highest tolerance for both acid and bile tests. Sequencing of 16s rRNA using primers FC27 sense and RC1492 antisense revealed that PAR5 (99%), NN39 (96%) and LK13 (99%) are homologous to Pediococcus pentosaceous ATCC 25745. LK13 exhibited the highest Betha-galactosidase activity (5.15x10-6 UoNPG/mg biomass). The developed anti-diarrheal product with freeze-dried ikmo extract and Par5 (10 sup 9 cfu/mL) were non-infected with common food pathogens and able to inhibit these pathogens in vitro, even after 8 weeks of storage whether at refrigerated o r freezing temperatures. Test for acute oral toxicity of the product showed LD50 at 27.97g/kg. Anti-diarrheal test using laboratory animals is recommended to confirm functionality.

Language

English

Document Type

Article

Pages /Collation

24 leaves

En – AGROVOC descriptors

MOMORDICA CHARANTIA; LEAVES; PLANT EXTRACTS; PIPER BETLE; INDIGENOUS ORGANISMS; PROBIOTICS; LACTIC ACID BACTERIA; ANTIMICROBIAL PROPERTIES; DRUG PLANTS; HERBACEOUS PLANTS; MEDICINAL PROPERTIES

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