Efficacy of leaf extracts from robusta coffee (Coffea canephora) on the larval and pupal development of fall armyworm (Spadoptera frugiperda)

Date

2025

Adviser

Lea C. Garcia

Principal

Mabel S. Buela

Abstract

Robusta coffee (Coffea canephora) leaves, known for their phytochemical content, were used to prepare a crude extract called Robusta Leaf Extract (RLE), which was previously found to have antibacterial and possible insecticidal activity. This study evaluated the efficacy of RLE on inhibiting the development of the larval and pupal life stages in Spodoptera frugiperda, along with its dose-response relationship. S. frugiperda or fall armyworm (FAW) poses a serious threat to rice and maize production in the Philippines. In the experiment, samples were fed an artificial diet mixed with RLE across four concentrations (1, 3, 5, and 10 mg/g), with a separate group serving as control. Key parameters measured were larval mass, pupal mass, and pupal count, which were then analyzed using JAMOVI’s One-way analysis of variance test (ANOVA). At the 5% level of significance, results showed a dose-dependent reduction in mean larval mass, with a statistically significant decrease observed at 3 mg/g (186.48 mg), 5 mg/g (144.30 mg), and 10 mg/g (78.61 mg) compared to the control. However, pupal mass and pupal count showed no clear dose-response trend, with 5 mg/g yielding the lowest final pupal count, and the only significantly lower mean pupal mass (174.29 mg) compared to the control. Meanwhile, the higher concentration group at 10 mg/g showed signs of possible tolerance. The sublethal effective dose (SED) was identified at 5 mg/g. These findings suggest that RLE has potential as a safe, cost-effective, and locally available alternative to commercial pesticide use.

Language

English

Location

UP Rural High School

Notes

To access this capstone, please contact the UP Rural High School Library at uprhslibrary.uplb@up.edu.ph. You may also visit the library in person, provided you secure prior confirmation from the librarian. We will be happy to assist you.

Document Type

Capstone

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