A study on the identification of possible Phytochemical constituents and the Antioxidant potential of Kapa-Kapa (Medinilla magnifica Lindl.) Leaf extracts.

Date

6-2018

Academic Strand

Science, Technology, Engineering, and Mathematics (STEM) Strand

Adviser

Ruby Lynn G. Ventura

Principal

Liza C. Carascal

Abstract

Shrubs, in general, are potential medicinal plants that are reported to have pharmacological properties. Specifically, some shrubs in the Philippines are among the untapped sources for potential phytochemicals. The goal of this is to identify the possible phytochemical constituents and antioxidant potential present in crude and fractionated extracts of the leaves of an endemic shrub in the Philippines, the kapa-kapa (Mdenilla magnifica Lindl.). The specific objectives were (a0 to prepare a crude methanolic extract from the leaves of Kapa-kapa (medinilla magnifica Lindl.), (2) ro produce fractions of the crude methanolic extract of M. magnifica Lindl. leaves using column chromatography with n-hexane and ethyl acetate as solvents. (3) to identify the possible compounds present in the produced crude and fractionated extracts using Liquid Chromatography-Mass Spectrometry (LC-MS), (4) to determine the anti-oxidative activity of the crude and fractionated extracts using 2,2 assay, and (5) to identify the M. magnifica Lindl. leaves crude and fraction with the highest antioxidant activity using 2.2-Diphenyl-1Picrylhydrazyl (DPPH) assay, Liquid Chromatography - mass Spectroscopy (LC-MS) identified candidate compounds which are mostly alkaloids and flavonoids from the crude and fractionated extracts. All Kapa-kapa leaf extracts were found to have antioxidant property through DPPH assay. Among the anti-oxidative, triprolidine belongs to the alkaloid group while quercetin, linarin, and fortunellin belongs to the flavonoid group. This study served as an exploratory work that may lead to identification of phytochemical found in the leaves of Kapa-kapa, and possible bioactivities that are promising in the medical field.

Language

English

LC Subject

Capstone

Location

University of the Philippines Rural High School

Document Type

Capstone

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