Research-internship at the CEAT Biomaterials and environmentak engineering laboratory with a special problem on: inverstigation of the biohydrogen production potential of an encapsulated Rhodobacter sp. local isolate, May 2

Date

6-2023

Academic Strand

Science, Technology, Engineering, and Mathematics (STEM) Strand

Adviser

Ruby Lynn G. Ventura

Principal

Mabel S. Buela

Abstract

The energy crisis that the world is currently facing calls for drastic measures as the pace at which fuels are consumed has now risen to an alarming rate. With this, the objective of the research internship is to learn basic laboratory skills and apply these in a mini-problem regarding alternative energy production. In collaboration with College of Engineering and Agro-Industrial Technologies Biomaterials and Environmental Engineering Laboratory (BEEL), the researchers did some work immersion to familiarize themselves with the different equipment and techniques they’ll use and form a mini-investigative study to address these current issues. In this mini study, the researchers aimed to produce hydrogen via sustainable means by implementing immobilization through encapsulation of Rhodobacter sp. local isolate MAY2. The immobilization of local isolate MAY2 was carried out using the different encapsulation matrices: sodium alginate (SA), agar, and sodium alginate-polyvinyl alcohol SA-PVA). Different concentrations of these materials were tested for transparency and firmness; and 1.5% SA, 0.75% SA 5% PVA, and 1.875 g/250 mL agar were identified to be ideal for use in the capsulation of the strain. The potential of the encapsulated inoculum to produce hydrogen was investigated using the following parameters: optical density at 660 nm, volume of hydrogen produced, and pH. Out of these, agar-encapsulated PNSB was found to produce the highest amount (27.326%) of accumulated hydrogen which was followed by SA (15.3805%). SA-PVA was found to be ineffective as it gave the smallest (1.353%) cumulative of H2. All of the encapsulated setups were found to have a possible matrix rupture because they were found to have OD values similar to the control., especially with the culture broth of agar encapsulated MAY2 as its OD has a difference of 0.500.

Language

English

LC Subject

Capstone

Location

University of the Philippines Rural High School

Document Type

Capstone

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