Genetic variability of banana bunchy top virus and abaca bunchy top virus in abaca (Musa textilis Nee,)

Date

6-2018

Abstract

The abaca bunchy top has been previously identified to be caused by Banana bunchy top virus (BBTV) and Abaca bunchy top virus (ABTV). In this study, the genetic variability of BBTV and ABTV was determined through genomics approach. Virus isolates from a total of nine locations in Bicol, Visayas, and Mindanao were analyzed. The restriction enzyme digestion of rolling circle amplified DNA (RCA-RFLP) has indicated variability between the Bicol isolates, and isolates from Visayas and Mindanao. The DNA- U3 is most variable among the component genomes with eight variations detected by restriction mapping in the virus genomes. Sequence analyses revealed that Bicol isolates are ABTV while Visayas and Mindanao isolates are BBTV. Within species, the ABTV isolates have 99.5% nucleotide identities while BBTV isolates have 93 to 100%, indicating very low genetic variability. Between species, the BBTV and ABTV have 66 to 79% nucleotide identities. Phylogenetic relationship analysis divided the virus isolates into ABTV group in Bicol and BBTV group in Visayas and Mindanao. Some recombination evidences were detected in DNA-U3, DNA-S, DNA-R, and DNA-C of isolates L9-2 (Legaspi), SSS (Surigao del Sur), K13 (Kananga), and B7 (Baybay), respectively. The knowledge generated from this study has important implication in developing, screening and deploying of resistant genotypes to manage abaca bunchy top.

Document Type

Dissertation

Degree

Doctor of Philosophy in Plant Pathology

College

Graduate School (GS)

Adviser/Committee Chair

Filomena C. Sta. Cruz

Committee Member

Christian Joseph R. Cumagun, Antonio G. Lalusin, Maria Genaleen Q. Diaz

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

En – AGROVOC descriptors

MUSA TEXTILIS; GENOTYPES; BANANA BUNCHY TOP VIRUS; GENETIC VARIATION; PHILIPPINES

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