Functional validation of the drought-responsive rice transcription factor "no apical meristem" (OsNAM12.1)
Date
6-2018
Abstract
The rice gene for transcription factor No Apical Meristem (OsNAM12.1) plays a central role in rice yield under drought through a network of co-localized genes at the QTL qDTY12.1. In this study, OsNAM12.1 was demonstrated to effectively transactivate a reporter gene via transient expression assays in rice and tobacco protoplasts. Transactivation activity of OsNAM12.1 using a yeast system was not successful in earlier studies. However, transactivation in a plant-based system suggests that plant-specific protein post translational modifications (PTMs) may be essential for this function. The OsNAM12.1 PTM, as profiled by 1-dimensional immuno-detected bands showed differences in band pattern between three rice varieties Vandana, Way Rarem and the near isogenic line (NIL) 481-B under well-watered and drought conditions. The differential band pattern suggested that the in silico predicted PT of SUMOylation phosphorylation and/or oligomerization may underpin these differences. In addition to transactivation and PTM studies, expression analysis in IR64+qDTY2.3 and IR64+qDTY12.1/2.3 near isogenic lines showed that OsNAM12.1 is upregulated two-fold under drought. Thus suggesting that OsNAM12.1. may be transcriptionally regulated by a gene or a set of genes found in qDTY2.3. Preliminary expression analyses exhibited that some qDTY2.3 putative candidate genes may indeed have a role in transcriptional regulation of OsNAM12.1. Further analyses have showed a MAPKK gene to be the most probable candidate gene responsible for such regulation of OsNAM12.1.
Document Type
Dissertation
Degree
Doctor of Philosophy in Genetics
College
Graduate School (GS)
Adviser/Committee Chair
Merlyn S. Mendioro
Co-adviser
Ajay Kohli
Committee Member
Maribel L. Dionisio-Sese, Ma. Genaleen Q. Diaz
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Recommended Citation
Trinidad, Jennylyn L., "Functional validation of the drought-responsive rice transcription factor "no apical meristem" (OsNAM12.1)" (2018). Graduate Student's Output. 2915.
https://www.ukdr.uplb.edu.ph/etd-grad/2915
En – AGROVOC descriptors
ORYZA SATIVA; GENES; APICAL MERISTEMS; TRANSCRIPTION FACTORS; FUNCTIONAL ANALYSIS; DROUGHT STRESS