Comparative study of six alternative solutions for embalming dogs for gross dissection and fixatives for histological studies

Date

6-2018

Abstract

This study was conducted to compare the efficiency of six alternative fixative solutions with formalin fixation of dog specimen for gross and histological studies.

Thirty-five non-descript dogs with the body condition score range of 3-5 out of 1-9 were used and euthanatized via the common carotid artery. They were divided into seven treatment groups with five animals per treatment by stratified sampling. Animals in each treatment group were embalmed with formaldehyde (TI), Soap Ethanol-based Fixing Solution (T2), Weigner's Solution (T3), modified SEFS 1 (T4), modified SEFS 2 (T5), modified Weigner's Solution 1 (T6) and modified Weigner's Solution 2 (T7). Color, texture, haptic property and conjunctival irritation scoring was done once a month for five months. Bacterial and fungal isolation was conducted to determine the presence of microbes. One non-descript dog was used for histological study and tissue samples were collected, fixed with the six alternative solutions and formalin solution, then subiected to routine paraffin technique at 24 hours, 48 hours and 72 hours post-fixation.

Document Type

Master Thesis

College

Graduate School (GS)

Adviser/Committee Chair

Bella C. Cruzana

Committee Member

Ceferino P. Maala, Rio John T. Ducusin

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

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