Segregation pattern of peroxidase isozymes in three oryza species: sativa, nivara and rufipogon

Date

7-1980

Abstract

The segregation pattern of peroxidase isozymes was studied in two wild rice species Oryza nivara and O. rufipogon and in hybrid populations in their crosses with cultivated rice O. sativa using polyacrylamide disc gel electrophoresis.

A total of eight peroxidase bands were observed on the parents and two new bands, Px1 Px3 and Px1 Px4, from the F1 and F2 progenies. Peroxidases 1 (Px1) and 6 (Px6) were found to be common to all species.

A hybrid isozyme Px1 Px4 was observed in the hybrids of O. nivara (Acc no 101524) and O.rufipogon (Acc no. 101448) with IR 42. Based on F2 segregation, Px1Px4 appeared to be a product of the interaction between genes Px1 of O. sativa (IR 42) and Px4 of O. nivara (Acc no. 101524) and O. rufipogon (Acc no. 101448). The isozyme Px1Px3 must have resulted from an interaction between gene Px1 of IR 42 and Px3 of O. rufipogon (Acc no. 100960).

Isozymes coded by genes Px1, Px3 and Px4 were presumed to be dimeric. Allelism and codominance of these genes could produce a hybrid isozyme in the heterozygotes, a hypothesis supported by the F2 which showed a good fit to the expected 1 (Px1):2 (Px1-Px1Px4-Px4):1 (Px4) or 1 (Px1):2 (Px1-Px1Px3-Px3): 1 (Px3) segregation ratio.

Document Type

Master Thesis

Degree

Master of Science in Agronomy

Major Course

Major in Agronomy

College

Graduate School (GS)

Adviser/Committee Chair

Tomas M. Masajo

Co-adviser

Dolores A. Ramirez

Committee Member

Mariano B. De Ramos

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 995 1980 A42 S65

DOI

https://www.ukdr.uplb.edu.ph/cgi/ir_submit.cgi?context=etd-grad&edbypass=1&editpanel=

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