Morphometrical, cytological, and biochemical characterization of tungro-transmitter and tungro-nontransmitter populations of the green leafhopper, Nephotettix virescens (Distant)

Date

10-1998

Abstract

This study analysed and compared morphometrical, cytological, and biochemical variations in N. virescens tungro-transmitter and tungro-nontransmitter populations.

Discriminant analyses of morphometrical and cytological characteristics were employed to construct models that had greater than 50% probability of correctly classifying tungro-transmitters and tungro-nontransmitters.

Morphometrical investigations of ethanolfixed and KOH-cleared male adult N. virescens showed that tungro-transmitters had significantly greater values over tungronontransmiters for head width, long stylet length, penultimate rostral segment width, ultimate rostral segment width, ultimate rostral segment length, and vertex length and width.

Lacto-aceto-orcein squashes of testicular tissue of N. virescens revealed that tungro-transmitters had significantly higher values for leptonema and telophase I nuclei width, transmitters had greater relative chromosomes length for chromosomes I, V, IV, VIII, however tungro-nontransmitters had higher relative chromosome lengths for chromosomes II, III, VI, VII.

Electrophoretic analysis of genetic variation at seven enzyme loci showed that six were polymorphic and one was monomorphic. Hardy-Weinberg equilibria were obtained in three loci in tungro-transmitters, but only two in tungro-nontransmitters. Proportion of polymorphic loci, average number of alleles, and heterozygosity were similar in tungro-transmitters and tungro-nontransmitters. High genetic identity and low genetic distance indicated that both types of N. virescens belong to a single species.

Document Type

Dissertation

Degree

Doctor of Philosophy in Genetics

College

Graduate School (GS)

Adviser/Committee Chair

Merlyn S. Mendioro

Committee Member

Nieva L. Basilio, Florinia E. Merca, Rita P. Laude

Language

English

LC Subject

Leafhoppers

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 996 1998 G2 M35

Notes

Doctor of Philosophy (Genetics)

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