Evaluation of the occurrence of familial hypercholesterolemia among Filipinos in the Philippines using and molecular genetic approaches
Date
10-1998
Abstract
The occurrence of familial hypercholesterolemia (FH) among Filipinos in the Philippines was evaluated using clinical and molecular genetic approaches. The clinical approach was based on the cholesterol profile of the prospective subjects of the study to identify suspected FH individuals together with other factors such as age and gender, diet and lifestyle, and the occurrence of cardiovascular disease among the subjects. The molecular genetic approach used was the PCR-based single strand conformation polymorphism (SSCP) analysis which may allow the molecular identification of familial hypercholesterolemic patients, and thus, may become a diagnostic tool for FH condition. The study initially surveyed some 37,500 patients from the blood chemistry records of the cooperating hospitals. From these patients, 332 were found hypercholesterolemics based on their total serum cholesterol. From the 332, some 75 individuals were suspected with familial hypercholesterolemia based on their LDL and HDL cholesterol levels, and based on their blood sugar and creatinine levels within the normal range. The final population of the study was 64 as 11 were deceased. It found that among the 64 suspected FH, the incidence of hypercholesterolemia was higher among males than females especially in age group 30-40. The rise of serum total and LDL cholesterol levels goes with age as observed among ages 50-60 where mean cholesterol were highest. About 52% of the subjects showed manifestations of cardiovascular disease or any hypercholesterol-related disorder. Also 33% of them were vegetarians, 78% nonsmoker/nondrinkers, and 28% were engaged in regular fitness program. Age showed a significant relationship with total and LDL cholesterol levels. The increase of total and LDL cholesterol levels, and the incidence of cardiovascular disease was found related with age. Based on the occurrence of cardio vascular disease (CVD), the total pedigree score of families with suspected FH subjects ranged from 8-35 which does not necessarily reflect the occurrence of FH in the family but rather the occurrence of CVD among family members of each suspected FH patients. The SSCP analysis was not found conclusive because of the observed extra bands need to be further verified. Nevertheless, the study laid the first attempt to use SSCP as a molecular genetic approach in the evaluation of FH under local conditions.
Document Type
Dissertation
Degree
Doctor of Philosophy in Genetics
College
Graduate School (GS)
Adviser/Committee Chair
Rita P. Laude
Co-adviser
Saturnina C. Halos
Committee Member
Madeline M. Suva, Merlyn S. Mendioro, Desiree M. Hautea
Language
English
LC Subject
Hypercholesterolemia
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
LG 996 1998 G2 T35
Recommended Citation
Tamayo, Jerry S., "Evaluation of the occurrence of familial hypercholesterolemia among Filipinos in the Philippines using and molecular genetic approaches" (1998). Graduate Student's Output. 3335.
https://www.ukdr.uplb.edu.ph/etd-grad/3335
Notes
Doctor of Philosophy (Genetics)