Development of simultaneous lysine production and separation process from agricultural substrates by membrane filtration

Date

1992

Abstract

Membrane process for separation of lysine by dynamic membrane system was evaluated. Dynamic membrane formed from polyion complex filter exhibited the highest rejection value (R) of 0.27 for lysine. Separation process by perstraction was studiedusing Polyvinylalcohol (PVA) membrane and Zeolite ZCP 50 as ion exchange resin. Rejection value (R) of 0.57 was measured at lysine concetration of 210 mM. Equilibrium isotherm experiment was also done to estimate the cation exchange capacity (Q0) and equilibrium coefficient (Ke) for Zeolite ZCP 50. Results showed the Q0 (2.08 mole/kg) and Ke (2.43 mole/m^3) for ZCP 50. Production of lysine by threonine auxtroph mutant of Corynebacterium glutamicum (th 12) was done by batch fermentation process. Kinetics of fermentation process were studied using three agricultural substrates: (a) molasses, (b) brown sugar and (c) sugarcane juice. Molasses produced the highest lysine yield, 37.95 g/l at 60 h fermentation time. Conversion ratio of 0.39% was attained based on total sugar level. Continuous lysine production with cell recycling was studied using molasses as substrate. Highest lysine yield of 41.43 g/l was attained at 1 ml/min feed rate. Lysine production beyone 120 h was done with no significant decrease in yield. A design of continuous lysine production coupled with membrane separation process. Using 0.53 ml/min feed rate with molasses as substrate lysine produced from fermentation process was separated by the membrane system of PVA and Zeolite ZCP 50. Lysine was removed at a rate of 0.36 glys/h.

Document Type

Master Thesis

Degree

Master of Science in in Agricultural Engineering

Language

English

LC Subject

Lysine

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 995 1992 A2 A96

This document is currently not available here.

Share

COinS