Characterization and identification of bacteria isolated from mine tailing-contaminated soil and their potential in promoting plant growth in copper-contaminated soil / Erny Yuniarti ; Ida F. Dalmacio, chair, advisory committee.

Date

6-2017

Abstract

Lead (Pb), cadmium (Cd), and copper (Cu) content level of soil at the gold mine tailing dam of PT. ANTAM in Pongkor, Bogor, Indonesia was within the permitted level of normal soil while soil at the inactive mine site in Mogpog, Marinduque, Philippines was contaminated with 446.3 ppm of Cu which is above the 36 ppm allowable Cu content in soil. Most of the isolated soil and rhizosphere bacteria from the Cu mined-out site and its tailing dam (66.7% from 21 isolates) were resistant to Cu while most of the rhizosphere bacteria from the gold mine site (77.8% from 18 isolates) were sensitive to 72 ppm Cu. The isolated bacteria from both sites showed high resistance to lead and cadmium wherein the latter was more toxic than the former. Three (7.7%) of the heavy metal-resistant bacteria were able to form biofilm and like the other heavy metal-resistant bacteria (91.4% from 35), they showed one or several of the growth promoting characteristics but not biofilm formation. Similarity analysis of 16S the rDNA sequences, indicated the 19 representative bacteria as members of four phyla, namely: Proteobacteria (Alphaproteobacteria and Gammaproteobacteria), Firmicutes, Bacteriodetes, and Actinobacteria. Heavy metal-resistant bacteria, CuNFbM 4.1 (Fulvimonas yonginensis) and MGR 333 (Rhizobium sp.) were used as Inoculant A (multiple heavy metal resistant, N fixer, phosphate solubilizer, IAA producer) and CuNFbM 4.1, MGR 333, and PbSM 2.1 (Pseudomonas marginalis) as Inoculant B (multiple heavy metal resistant, N fixer, phosphate solubilizer, IAA and ACC deaminase producer). Lime (2 tons/ha), complete fertilizers (80 kg/ha urea, 300 kg/ha SP-36, 150 kg/ha KCl) and compost amendment were used as positive control in assessing the capability of the inoculants to assist narra growth and its Cu accumulation under greenhouse experiment. All treatments, i.e., Cu contamination, lime together with complete fertilizer, compost and inoculant resulted to significant differences in the stem diameter of narra. Narra could thrive in Cu mine waste-degraded soil with PGPB inoculation and addition of lime with complete fertilizer or 4% compost. The contribution of plant growth-promoting bacteria (PGPB) inoculation to plant growth is not comparable to chemical fertilizers. However, with PGPB inoculation, there is reduction in fertilizer and compost application. Various doses of fertilizer and/or compost combined with inoculant, and different levels of compost less than 4% (the recommended amount in agriculture, 2 tons/ha or 0.1%) can be evaluated. The reduction in the volume of compost which is bulky, will be an additional help to personel involved in phytoremediation application.

Document Type

Dissertation

Degree

Doctor of Philosophy in Microbiology

Major Course

Major in Microbiology

College

Graduate School (GS)

Adviser/Committee Chair

Ida F. Dalmacio

Committee Member

Asuncion K. Raymundo, Virginia C. Cuevas, Erlina S. Paterno, Nina M. Cadiz

Language

English

LC Subject

Bacteria -- Identification, Plant regulators, Growth (Plants), Plants -- Effect of pollution on, Plant growing media -- Contamination, Soil pollution, Microbiology Institute of Biological Sciences

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 996 2017 M5 E76

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