Molecular breeding for simultaneous improvement of drought and salinity tolerance in rice / Muhammad Umair Aslam ; Jose E. Hernandez, chair, advisory committee.

Date

6-2017

Abstract

A dissertation was carried out to study simultaneous improvement for drought and salinity tolerance in rice utilizing molecular markers. Three early backcross introgression populations in BC1F5 generation in Weed Tolerant Rice1 recipient background derived from 3 different donors i.e. Haonnong, Cheng-Hui448 and Basmati-385 were screened and phenotyped under irrigated, drought and salinity conditions for four cropping seasons (2014WS, 2015DS, 2015WS, and 2016DS). Best performing introgression lines in terms of yield performance were identified in target environments. All three sub-populations were genotyped using tunable genotyping by sequencing for SNP-typing and identification of chromosomal introgression patterns. Field and glasshouse phenotyping and genotyping results were used as basis for QTL mapping analysis to identify genetic basis of tolerance to drought and salinity. 43 significant major and 956 minor QTLs were identified and with up to 41.96% of significant genetic overlaps for drought and salinity tolerance were detected. The novel QTL resources mapped in this study are important in dissecting the genetic basis of drought and salinity tolerance in rice. The SNPs/QTLs mapped will be useful in designing array based screening for marker-assisted selection.

Document Type

Dissertation

Degree

Doctor of Philosophy in Plant Breeding

College

Graduate School (GS)

Adviser/Committee Chair

Jose E. Hernandez

Committee Member

Pompe C. Sta. Cruz, Antonio G. Lalusin, Jauhar Ali, Jose E. Hernandez

Language

English

LC Subject

Crops, Rice, Plant Breeding College of Agriculture and Food Science

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 996 2017 A42 A85

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