Selection, characterization and infectivity of entomopathogenic fungi against the spider mite Tetranychus kanzawai (Kishida) (Tetranychidae: Acarina)/ Yayan Sanjaya ; Virginia R. Ocampo, chair, advisory committee.

Date

6-2011

Abstract

Six isolates of Beauveria bassiana (Bb1 to Bb6), seven isolates of Metarhizium anisopliae (Ma1 to Ma7), and one isolate of Paecilomyces lilacinus (P1) from Philippines and Indonesia were evaluated for their infectivity to Tetranychus kanzawai (Kishida). The selected three isolates each of Bb and Ma were pathogenic to mite with LC50 value of 1.2 X 10^3 to 2.4X10^3, 5.0 X 10^2 to 1.3 X 10^3 for Ma after days post infection under laboratory condition. When 10^8 concentrations of the seven isolates were sprayed to papaya seedlings in the greenhouse, LT50 values ranged from 3.0 to 4.2 days for Ma and 3.98 to 5.48 days for Bb. Pl was not infective to T. Kanzawai compared to the other two fungal pathogens. Characterization using morphological and molecular analyses showed that selected Ma, Bb and P1 entomopathogenic isolates virulent against T. Kanzawai belong to B. bassiana, M. anisopliae, and P. lilacinus species, respectively. ITS DNA sequence analyses also indicated variation within Beauveria and Metarhizium isolates.

Document Type

Dissertation

Degree

Doctor of Philosophy in Entomology

College

Graduate School (GS)

Adviser/Committee Chair

Virginia R. Ocampo

Co-adviser

Barbara L. Caoili

Committee Member

Nicomedes D. Briones, Pio A. Javier, Barbara L. Caoili, Virginia R. Ocampo

Language

English

LC Subject

Fungi, Fungi, Insect nematodes, Entomology Crop Protection Cluster

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 996 2011 E75 Y39

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