Development of nutrient use efficient (nue) rice (Oryza sativa L.) cultivars and identification of QTLs governing nue related traits / Zilhas Ahmed Jewel ; Jose E. Hernandez, chair advisory committee.

Date

7-2015

Abstract

The author used the IRRI-GSR nutrient use efficiency protocol to first identify suitable donor and recipient parents and later derived 230 NUE-introgression lines in Weed Tolerant Rice 1 recipient parental background introgressed with donor alleles from Haoannong that were systematically screened in field trials conducted from dry season (DS) 2011 to DS 2014 across six different nutrient conditions (+NPK, 75N, -N, -P, -NP and ûNPK) in relation to the Site specific nutrient management recommended fertilizer levels. First round of screening of BC sub 1 F sub 2 bulk populations were conducted underrainfed-drought, low input and irrigated conditions to select for stay green, robust stature, heavy tillering and high yielding single plant selections. Desirable 230 NUE lines were developed from these selections by three successive rounds of screening across six nutrient use efficient conditions. First round involved the screening of 4 BC sub 1 F sub 3 populations derived in Weed Tolerant Rice 1 (WTR1) recipient parental background from 4 donor parents i.e. Haoannong, Zhong413, Y134 and BG300. Out of these 4 populations only one i.e. WTR1*2/Haoannong population was selected for the next two rounds of screening to identify 230 nutrient use efficient introgression lines based on yield and yield component traits as compared to the parents and national check varieties i,e, PSB Rc82 and NSIC Rc 222. Five most promising IRRI bred NUE GSR lines identified (GSR IR2-1-RF6-NU4-NU7-NU70,GSR IR2-1-RF3-NU2-NU2-NU3- GSR IR2-1-RF6-NU4-NU10-NU29, GSR IR2-1-Y16-NU1-NU1-NU4 and GSR IR2-1-Y17-NU2-NU1-NU8) were for systematic evaluation under greenhouse potted experiment with six varying levels of SSNM that clearly showed the higher NPK (166.5 and 133.2%) could increase the grain yields. However, at optimum SSNM (99.9%) levels most of the Introgression lines had shown relatively good performance. Interestingly at 66.6% of SSNM the yields were compatible with 99.9%, and this shows promise that by reducing fertilizers by 33.3%, the author could still get comparable yields to that of 99.9%. Likewise, these newly developed ILs also showed that even by additional dosage of 33.3% of fertilizers the author are not going to make significant increase in yields over 99.9% SSNM levels. It also confirms that screening protocol and selection strategy are working well to identify NUE-ILs materials. A total of four new putative QTLs (qAE_2.1, qAE4_.1, qAE_6.1, and qAE_12.1) were detected for agronomic efficiency on chromosome 2, 4, 6 and 12 respectively, where agronomic efficiency was determined as AE sub(NK)-phosphorus deficient soil and AE sub (75N)-applied 75% nitrogen nutrient conditions using single marker analysis (SMA) method with a LOD value range 2.55-4.52 and phenotypic variation explained (PVE) range of 5.92 to 10.27%. For partial factor productivity (PFP) trait, the author discovered twelve new put ative QTLs (QTLs qPFP_1.1, qPFP_2.1, qPFP_3.1, qPF_4.1, qPFP_5.1, qPFP_5.2, qPFP _6.1, qPFP_7.1, qPFP_8.1, qPFP_9.1, qPFP_10.1, qPFP_11.1) that were detected on chromosome 1, 2, 3, 4, 5, 6, 7, 8,9, 10 and 11 respectively only under PFP sub (NK)- phosphorus deficient soil and PFP sub (75N) sup - applied 75% nitrogen conditions with a LOD value range 3.39-12.15 and PVE range of 7.81 to 25.8%. Significant SNP markers SNP_9_482477, SNP_11_27994133 and SNP_3_851785 were detected for agronomic traits like plant height, days to flowering and days to maturity under 75N, + NPK and P conditions respectively. Significant peak markers SNP_5_18190785, SNP_3_35422519, SNP_8_23719048, SNP_9_112154616, SNP_4_23320234, SNP_5_6506422, and SNP_3_35422519, SNP_8_23719048, SNP_9_112154616, SNP_4_23320234, SNP_5_ 6506422 and SNP_3_35422519, SNP_8_23719048, SNP_9_112154616, SNP_4_23320234, SNP_5_6506422 and SNP_3_853802 were detected for morphological traits like effective tiller number per plant, panicle length, flag leaf length, flag leaf width, and flag leaf area under -N, 75N and -P conditions respectively. Likewise for phys iological traits, significant peak markers SNP_3_30247826, SNP_10_1820606, SNP_2_4481943 and SNP_11_22440795 were detected for SPAD reading, percentage of spikelet fertility and biomass yield under +NPK, -N-P, conditions respectively.

Document Type

Dissertation

College

Graduate School (GS)

Adviser/Committee Chair

Jose E. Hernandez

Co-adviser

Jauhar Ali

Committee Member

Jose E. Hernandez, Jauhar Ali, Teresita H. Borromeo, Antonio G. Lalusin

Language

English

LC Subject

Rice, Plant Breeding College of Agriculture and Food Science

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 996 2015 A42 J49

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