Molecular diversity of methanogens, nitrogen balance and blood metabolite levels in ruminants as influenced by dietary tannin / Gondelina Abuel Radovan ; Cesar C. Sevilla, chair, advisory committee.
Date
4-2011
Abstract
THe molecular diversity of rumen methanogens in domesticated carabao and cattle fed tannin - containing banana leaves or supplemented with commercial tannin extract was assessed using polymerase chain reaction - denaturing gadient gel electrophoresis (PCR-DGGE). Primer set 0357 F-GC and 0691 R was used to amplify the methanogenic archaeal community of the rumen. A total of 26 DNA fragments were excised from DGGE gels and their nucleotide sequences were successfully determined. PCR-DGGE band profile and nucleotide sequence analysis revealed that domesticated carabao harbor fewer methanogens compared to cattle. Methanogen resembling Methanobrevibacter sp. YE288 is the predominant methanogen in carabao while Methanobrevibacter thaueri strain CW and Methanobrevibacter millerae strain ZA-10 are the predominant ones in cattle. Feeding tannin - containing banana leaves remarkably altered the methanogen composition of both carabao and cattle more than commercial tannin extract supplementation in the diet. In addition, feeding of tannin - containing banana leaves to cattle significantly increased blood urea nitrogen (BUN) but decrease rumen fluid ammonia nitrogen (RF-NH3-N) level suggesting a protective effect of tannin on feed protein and possible inhibitory effect of ammonia on methanogens due to limited supply of hydrogen. However, overall nitrogen balance as well as nutritional status of animals remains unaffected.
Document Type
Dissertation
Degree
Doctor of Philosophy in Animal Science
College
Graduate School (GS)
Adviser/Committee Chair
Cesar C. Sevilla
Committee Member
Cesar C. Sevilla, Severino S. Capitan, Renato SA. Vega, Antonio J. Alcantara
Language
English
LC Subject
Ruminants, Carabao, Cattle, Tannins, Animal science Animal and Dairy Sciences Cluster
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
LG 996 2011 A5 R33
Recommended Citation
Radovan, Gondelina A., "Molecular diversity of methanogens, nitrogen balance and blood metabolite levels in ruminants as influenced by dietary tannin / Gondelina Abuel Radovan ; Cesar C. Sevilla, chair, advisory committee." (2011). Graduate Student's Output. 4211.
https://www.ukdr.uplb.edu.ph/etd-grad/4211