Morphological and molecular identification, optimum culture conditions and fructification, and biological activities of Lentinus sp. isolate 01 collected from Central Luzon, Philippines.

Date

12-2025

Abstract

Lentinus species (Polyporaceae) are saprophytic, wood-decaying basidiomycetes with remarkable nutritional and pharmacological properties. However, many wild species remain unexplored and underutilized, representing a missed opportunity to harness their full potential as a promising source of functional foods and bioactive compounds for medicine. This study generally aimed to determine the identity of Lentinus sp. isolate 01, establish optimum culture conditions for growth and determine the antimicrobial activity, and cytotoxicity against cancer cell lines. Based on morphological characteristics, Lentinus sp. isolate 01 was identified as Lentinus velutinus. However, BLASTn analysis revealed the nrDNA ITS of the Lentinus sp. isolate 01 has 99.65% similarity to GenBank database and 87% query cover to the nucleotide sequence of Lentinus sp. isolate L17 (OP522028.1) Phylogenetic analysis revealed that Lentinus sp. isolate 01 is distinct from Panus. The optimum mycelial growth was observed on potato dextrose agar, at pH 5, at 32°C, for 24 hours total dark, on sealed condition and grow on cracked corn seeds. The 7:3 formulation recorded the fastest mycelial and primordial initiation, highest total fruit yield per bag and biological efficiency percentage. Ethanolic extract of Lentinus sp. did not xhibit antimicrobial activity against E. coli, S. aureus, P, aeruginosa, B, subtilis, C. tropicalis and A. niger, and no cytotoxic activity against three acute myeloid leukemia (AML) cancer cells, OCI-AML3, MV4-1 1 and MOLM14. The growth characteristics of Lentinus mycelia differ among species and isolates, and can be improved under optimized culture conditions, which is critical for their nutraceutical, pharmaceutical, and industrial applications. Comprehensive evaluation of cytotoxicity and other bioactivities of Lentinus mycelia extracts is therefore strongly encouraged.

Document Type

Master Thesis

Degree

Master of Science in Microbiology

College

Graduate School (GS)

Adviser/Committee Chair

Rina B. Opulencia

Co-adviser

Rich Milton R. Dulay

Committee Member

Angella Melissa A. Carlos, Johnny F. Balidion

Language

English

En – AGROVOC descriptors

LENTINUS; BASIDIOMYCOTA; ANTIMICROBIAL PROPERTIES; CYTOTOXICITY; FUNGAL MORPHOLOGY; PHYLOGENETIC ANALYSIS

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