Marker-assisted gene stacking of drought, submergence, and rice tungro spherical virus resistance to Philippines rice varieties

Date

6-2024

Abstract

Drought, submergence, and rice tungro disease can cause substantial yield loss in rice around the world. Marker-assisted gene pyramiding is an important breeding strategy for conferring broad-spectrum and durable resistance to abiotic and biotic stresses into popular cultivars to reduce production loss and damage. The present study was carried out to improve the cultivars (PSB Rc14 and NSIC 2011 Rc286) through introgression of qDTY2.2 and qDTY4.1, Sub1 genes, and tsv1 resistance genes/QTLs by marker-assisted gene pyramiding. IR 102784-2-42-47-2-1-4 (qDTY2.2, qDTY4.1, Sub1) and IR 108963:6-1-1-3 (tsv1) were used as donors to introgress drought, submergence, and rice resistance into the recurrent parents (PSB Rc14 and NSIC 2011 Rc286). In the F2 generation, the improved pyramided lines carrying four, three, and two genes/QTLs combinations were selected. Digenic and trigenic QTL combinations identified in NSIC 2011 Rc286 PLs are qDTY2.2 + qDTY4.1 + tsv1, qDTY2.2 + qDTY4.1 + Sub1, qDTY4.1 + Sub1 + tsv1, qDTY2.2 + Sub1 + tsv1, qDTY4.1 + tsv1, qDTY2.2 + qDTY4.1, qDTY2.2 + Sub1, qDTY2.2 + tsv1, Sub1 + tsv1, and qDTY4.1 + Sub1. Whereas, the different QTL combinations recorded in PSB Rc14 PLs are qDTY2.2 + qDTY4.1 + Sub1 + tsv1, qDTY2.2 + qDTY4.1 + tsv1, qDTY2.2 + Sub1 + tsv1, qDTY2.2 + qDTY4.1 + Sub1, qDTY4.1 + Sub1 + tsv1, qDTY2.2 + tsv1, Sub1 + tsv1, qDTY2.2 + qDTY4.1, qDTY2.2 + Sub1, qDTY4.1 + Sub1, and qDTY4.1 + tsv1. Agronomic performance and the effect of different QTL combinations on yield and yield-related traits under non-stress conditions of the introgression lines (F2) with pyramided QTL/genes were also reported  in this study. The results showed that among the NSIC 2011 Rc286 complex F2 lines, a higher mean GY was recorded in the digenic line with qDTY4.1 + Sub1 combination. Meanwhile, the highest GY among PSB Rc14 complex F2 lines was measured in a trigenic line with qDTY2.2 + qDTY4.1 + tsv1 combination.

Document Type

Dissertation

Degree

Doctor of Philosophy in Plant Breeding

College

Graduate School (GS)

Adviser/Committee Chair

Jose E. Hernandez

Committee Member

Pompe C. Sta. Cruz, Antonio G. Lalusin, Teresita H. Borromeo

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

En – AGROVOC descriptors

ORYZA, DROUGHT, INTROGRESSION, GENE PYRAMIDING, RESISTANCE GENES, MARKER-ASSISTED SELECTION

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