"The diversity of the golden apple snail Pomacea canaliculata (Gastropoda: Proso -branchia) in the Philippines"

Date

2002

Abstract

Multiple introductions of the golden apple snail into the Philippines, Pomacea canaliculata, could indicate the presence of more than one species present in the country. To determine the diversity of the Pomacea canaliculata in the Philippines, morphological characterization and protein profiling of golden apple snails collected from eight provinces in the Philippines were conducted. Snail samples from Bukidnon, Cebu, Davao, Isabela, Leyte, North Cotabato, Nueva Ecija, and Nueva Vizcaya were compared and analyzed to determine whether or not the golden apple snail attacking rice in the Philippines belong to only one species.Protein extracted from the foot muscles of the collected Pomacea sp. were subjected to sodium dodecyl sulfate - polyacrylamide gel electrophoretic (SDS-PAGE) analysis. A total of four major protein bands were resolved with estimated molecular weights (MW) of 83, 40, 35, and 29 kDa. Three protein patterns emerged on the basis of the presence of each band : 1) Pattern A has all four major protein bands; 2) Pattern B has three major protein bands with MW of 83, 40, and 35 KDa; and 3) Pattern C has two major protein bands with MW of 83 and 35 KDa. In general, the protein pattern among snail samples from the samelocation did not show significant difference. However, across locations, major protein bands with MW of 83 and 35 KDa were consistently present while those with MW of 40 and 29 KDa were variable. Pattern A was manifested in snail samples from Davao; Pattern B was manifested in snail samples from Cebu, Isabela, Leyte, North Cotabato, Nueva Ecija and Nueva Vizcaya, and Pattern C was manifested in snail samples from Bukidnon. Electrophoretic fingerprints of snails belonging to one location are generally the same regardless of the variation in shell color and the prominence of the growth lines.The different protein patterns observed across locations may indicate genetic variability in the Pomacea canaliculata at the protein level. These proteins could be further characterized and may be used as genetic markers to come up with a more meaningful taxonomic classification of the Pomacea canaliculata in the Philippines.

Document Type

Master Thesis

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 995 2002 E8 F56

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