Tissue culture of Figus Benjamina L. 'variegata'

Date

6-1986

Degree

Bachelor of Science in Agriculture

Major Course

Major in Horticulture

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Helen L. Valmayor

Co-adviser

Aurora G. Del Rosario

Abstract

An acceptable method for surface sterilization of shoot tips with visible axillary buds was achieved from actively growing Ficus benjamins 'Variegata' plants preconditions for at least 2 months inside the greenhouse.

Short node sections, measuring from 0.3 -0.5 cm, with the lateral bud at the center of each section were induced to form and differentiate callus on a liquid culture medium containing MS salts and organic additives other than 2 - 3% sucrose with or without plant growth regulator supplements. Callus was initiated on explants treated with various levels and combinations of the cytokinins Ki and BA, and the auxins NAA and IAA, except at 5.0 mg/1 Ki + 0.2 mg/1 NAA. The number of days to callus initiation was reduced as the concentration of Ki and BA was increased from 0.5 to 5.0 mg/1.

A decreasing trend in the percentage and degree of callus formation was observed with increasing levels of Ki, BA and IAA. The presence of exogenous cytokinin and auxin at appropriate concentration and proportion prevented browning and eventual death of explants and calliformed, and promoted continuous production of callus with regular subculturing. Periods of incubation on the same nutrient medium greater than 4 weeks resulted in a definite decline in explant vigor.

The auxin NAA induced root formation in 60% of explants 21 days after inoculation while the combination of 1.0 mg/1 BA and 0.1 mg/1 IAA induced partial growth of lateral buds in 20% of the explants inoculated.

Two distinct types of callus were formed in certain combinations of Ki, NAA, BA and NAA or IAA, and in 0.5 mg/1 BA alone, while only one kind of callus was produced by explants treated with combinations of Ki, NAA or IAA only.

Plantlet formation was never observed.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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