Isozyme polymorphism in some Philippine native orchids: Denrobium anosmum Lindl., Dendrobium sanderae Rolfe, Cymbidium finlaysonianum Lind., and Cymbidium aliciae Quis.

Date

4-2006

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Carmina C. Manuel

Abstract

Isozyme polymorphism in Denrohium anosmum Lindl., D. sunderae Rolfe, Cymbidium finlaysonianum Lindl., and C. aliciae Quis was analyzed based on four enzyme systems, namely: acid phosphatase (ACPH), esterase (EST), malate dehydrogenase (MDH), and malic enzyme (ME) using starch gel electrophoresis. Isozyme banding patterns showed a total of seven presumptive loci. ACPH and EST exhibited the existence of several isoloci: three isoloci for EST, two for ACPH, and only one locus was noted for MDH and ME. The degree of genetic variability in the four species studied was determined by estimating the proportion of polymorphism (P), average allele per locus (A), average heterozygozity (H), genetic identity (IN), genotypic similarity (IH), and genetic distance (D). The P values of D. sanderae (20.00%) and C. aliciae (50.00%) were quite low compared to D. anosmum (83.33%) and C .finlaysonianum (71.43%). Also the H values of the four orchid species studied were below 50%. This shows that there is low genetic variability in the four species. Based on the enzyme loci studied, D. anosmum and C finlaysonianum had approximately two alleles per locus with an average of 1.774 while D. sanderae and C. aliciae had approximately one allele per locus with an average of 1.35. The computed estimates showed the least genetic identity (IN = 0.5650), lowest genotypic similarity (IH = 0.466), and the greatest genetic distance (D = 0.571) between D. sanderae and C jinlaysonianum.which could be due to genetic differences between the two genera. However, D. anosmum and C finlaysoniamun exhibited the greatest genetic identity (IN = 0.793), highest genotypic similarity (1H = 0.641), and the lowest genetic distance (D=232) even though they belong to different genera. It is possible that the variation between these two genera was not fully shown based on the four enzyme systems used.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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