Genetic diversity analysis of blast-, bacterial blight-, and tungro-resistant breeding lines of rice (Oryza sativa L.) using simple sequence repeat markers

Date

10-2008

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Joel C. Mendoza

Abstract

The genetic diversity of 104 blast-, bacterial blight-, and tungro-resistant breeding lines selected from annual reports by the International Network for Genetic Evaluation of Rice (INGER) was assessed using 50 polymorphic simple sequence repeat (SSR) markers. three hundred twelve alleles were detected with an average of 6.24 alleles per locus. Polymorphism information content (PIC) ranged from 0.29 to 0.89. The Dice coefficients of similarity of the 104 breeding lines were utilized for clustering analysis, which showed six groups at 0.38 level of similarity. Origin and percentage largely influenced the groupings of the breeding lines based on the dendogram. Furthermore, results showed that three was high variability among the selected breeding lines as these breeding lines began to diverge at 0.23 coefficient of similarity. This knowledge of genetic diversity could facilitate crop improvement by aiding in the planning of crosses that would ensure a broad genetic base and even hybrid vigor.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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