Karyotype analysis of in vitro cultured human leukocytes treated with sambong tablets Blumea balsamifera (L.) DC.
Date
3-1998
Degree
Bachelor of Science in Biology
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Merlyn S. Mendioro
Abstract
Leukocytes from two male donors were cultured and treated with sambong tablets of varying concentrations: 0% (control), 0.5%, 1.0% and 1.5%, which approximated the manufacturer's recommended daily dosage of the tablets. The chromosomes were arranged in a karyotype and their relative lengths were calculated. ANOVA was undertaken to determine the significance of the data. Results showed that the Chromosomes 1-6, 8-10 and 13 decreased in length while the rest increased. Chromosome 2 has the most negative slope (-0.302) indicating that it decreased in size the most while Chromosome 20 had the most positive slope (0.514) indicating that it had the greatest increase in length. Group A had the most negative slope (-1.048) while Chromosome X has the most positive slope (1.982). ANOVA shows that the results are not statistically significant to attribute the effects to the sambong tablets. The decreases in length is probably due to the camphor in the tablets that slowed the chromosome condensation.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
Thesis
Recommended Citation
Azote, Candy B., "Karyotype analysis of in vitro cultured human leukocytes treated with sambong tablets Blumea balsamifera (L.) DC." (1998). Undergraduate Theses. 10673.
https://www.ukdr.uplb.edu.ph/etd-undergrad/10673
Document Type
Thesis