Karyotype analysis of in vitro cultured human leukocytes treated with sambong tablets Blumea balsamifera (L.) DC.

Date

3-1998

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

Leukocytes from two male donors were cultured and treated with sambong tablets of varying concentrations: 0% (control), 0.5%, 1.0% and 1.5%, which approximated the manufacturer's recommended daily dosage of the tablets. The chromosomes were arranged in a karyotype and their relative lengths were calculated. ANOVA was undertaken to determine the significance of the data. Results showed that the Chromosomes 1-6, 8-10 and 13 decreased in length while the rest increased. Chromosome 2 has the most negative slope (-0.302) indicating that it decreased in size the most while Chromosome 20 had the most positive slope (0.514) indicating that it had the greatest increase in length. Group A had the most negative slope (-1.048) while Chromosome X has the most positive slope (1.982). ANOVA shows that the results are not statistically significant to attribute the effects to the sambong tablets. The decreases in length is probably due to the camphor in the tablets that slowed the chromosome condensation.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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