Detection of putative isoforms of Acetylcoenzymes A Carboxylase (ACCase) gene in coconut (Cocos nucifera L.).

Date

10-2003

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Co-adviser

Marni E. Cueno

Abstract

The putative isoforms of acetyi-coenzyme A carboxylase (ACCase) gene involved in the fatty acid synthesis in 4- 5- and 6-month-old coconut endosperms were determined using the 3' rapid amplification of cDNA ends (3' RACE) technique. The cDNA templates were amplified using two polymerase chain reaction (PCR) techniques: (1) cold-start and (2) combined hot-start and touchdown. Electrophoretic analysis of cold-start PCR products did not show bands in the 4-, 5- and 6-month-old coconut endosperms. This may be due to the formation of secondary structures caused by the GC rich region of the ACCase gene. Electrophoretic analysis of the combined hot-start and touchdown PCR products of the set-up with 43°C as annealing temperature showed faint bands while using 45°C as annealing temperature showed clear bands. Results showed that there are 8 putative isoforms in the 6-month-old coconut endosperm with approximately 300, 350, 400, 550, 650, 700, 850, and 975 by long; and 2 putative isoforms in the 4-month-old coconut endosperm with approximately 300 and 350 by long. This suggests that the gene is expressed at the 2 stages. However, no isoforms were generated from the 5-month-old coconut endosperm template suggesting that the gene is not expressed at this stage.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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