Cytogenetic effect of coffee on the salivary gland chromosomes of Drosophila melanogaster Meigen (Diptera: Drosophilidae).
Date
3-1990
Degree
Bachelor of Science in Biology
Major Course
Major in Cell Biology
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Adelina A. Barrion
Abstract
Populations of fruitflies were sampled at Umali Subdivision, Los Banos, Laguna. Investigations of the morphological characteristics such as the male sex comb (male genitalia) of the flies revealed that the predominating species collected was Drosophila melanogaster Meigen. Three brands of coffee namely: Great Taste (Brand A), Blend 45 (Brand B), and Nescafe Classic (Brand C) were introduced to the diet of D. melanogaster. This was done to determine the different cytogenetic effects of coffee on the polytene chromosomes of D. melanogaster. Polytene chromosomes excited tram the salivary glands of the third instar larvae of treated D. melanogaster showed the occurrence of several abnormalities such as chromosomal loops, chromosomal breaks, clumpings, circulars, and spreads. Based on the frequency of occurrence of these chromosomal aberrations, Brand C exhibited the highest count, followed by either Brands A or B. Statistical analysis using the Complete Randomized Design (CRD) showed that the treatments (coffee brands) significantly affected the polytene chromosome of D. melanogaster.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
Thesis
Recommended Citation
Arrienda, Charina Rose G., "Cytogenetic effect of coffee on the salivary gland chromosomes of Drosophila melanogaster Meigen (Diptera: Drosophilidae)." (1990). Undergraduate Theses. 10684.
https://www.ukdr.uplb.edu.ph/etd-undergrad/10684
Document Type
Thesis