Cytogenetic effect of coffee on the salivary gland chromosomes of Drosophila melanogaster Meigen (Diptera: Drosophilidae).

Date

3-1990

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Adelina A. Barrion

Abstract

Populations of fruitflies were sampled at Umali Subdivision, Los Banos, Laguna. Investigations of the morphological characteristics such as the male sex comb (male genitalia) of the flies revealed that the predominating species collected was Drosophila melanogaster Meigen. Three brands of coffee namely: Great Taste (Brand A), Blend 45 (Brand B), and Nescafe Classic (Brand C) were introduced to the diet of D. melanogaster. This was done to determine the different cytogenetic effects of coffee on the polytene chromosomes of D. melanogaster. Polytene chromosomes excited tram the salivary glands of the third instar larvae of treated D. melanogaster showed the occurrence of several abnormalities such as chromosomal loops, chromosomal breaks, clumpings, circulars, and spreads. Based on the frequency of occurrence of these chromosomal aberrations, Brand C exhibited the highest count, followed by either Brands A or B. Statistical analysis using the Complete Randomized Design (CRD) showed that the treatments (coffee brands) significantly affected the polytene chromosome of D. melanogaster.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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