Standardization of the screening technique for iron toxicity in solution culture system for rice (Oryza sativa L.).

Date

4-2006

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ivan Marcelo A. Duka

Abstract

Fifteen lines of rice (Orvza saliva L.) were screened during the seedling stage using twelve treatment combinations. The treatment factors were Fe(11) concentration (200 ppm, 300 ppm, and 400 ppm), pH (3 and 4.5), and presence of the chelator EDTA (0 ppm and 200 ppm). After two weeks of screening, visual symptoms were recorded. Bronzing was observed in all plants in all treatments. The more acidic the pH was and/or the more concentrated Fe was in the solution, the more severe the symptoms were noted in the plants. Using the indicator 1,10-phenanthroline in the colorimetric determination of Fe, the absorbance of each of the treatment solutions was read at 510 nm. In a period of six days, the Fe(II) concentrations of all treatments decreased by as much as 200 ppm. The original Fe concentration of the treatment and pH were found to be significant in causing the difference in the Fe(II) concentrations of the treatment solutions. EDTA was also found significant with respect to interacting with Fe concentration or pH. The recommended Fe concentration for the screening technique is 400 ppm. Addition of 200 ppm EDTA is also recommended and the pH should be 3.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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