Mutation for lysine overproduction in four strains of Saccharomyces cerevisiae

Date

10-1978

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ruben B. Aspiras

Abstract

Three thermotoleramt strains and the parent strain of Seccharomycks cerevisiae were mutated for methyllysine resistance following the tube dilution technique described by Scherr and Rafelson (1967). Only two strains SCW and SC61 were slightly inhibited by the presence of methyllysine in the medium. The remaining two strains SCE6 and SC89 were not inhibited even in the presence of 20 mg methyllysine/ml medium. Lysine production by the methyllysine resistant yeast strains and that of their respective parent strains were compared using a Presumable lysine requiring auxotroph of Micrococcus clutamicus as the test organism. No difference was observed in the degree of growth of the lysine auxotroph, whether it was streaked beside cells of the methyllysine resistant yeast strains or beside cells of the parent yeast strains. Further studies are needed to explain this observation.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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