Mutation for lysine overproduction in four strains of Saccharomyces cerevisiae
Date
10-1978
Degree
Bachelor of Science in Biology
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Ruben B. Aspiras
Abstract
Three thermotoleramt strains and the parent strain of Seccharomycks cerevisiae were mutated for methyllysine resistance following the tube dilution technique described by Scherr and Rafelson (1967). Only two strains SCW and SC61 were slightly inhibited by the presence of methyllysine in the medium. The remaining two strains SCE6 and SC89 were not inhibited even in the presence of 20 mg methyllysine/ml medium. Lysine production by the methyllysine resistant yeast strains and that of their respective parent strains were compared using a Presumable lysine requiring auxotroph of Micrococcus clutamicus as the test organism. No difference was observed in the degree of growth of the lysine auxotroph, whether it was streaked beside cells of the methyllysine resistant yeast strains or beside cells of the parent yeast strains. Further studies are needed to explain this observation.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
Thesis
Recommended Citation
Ardales, Edna Y., "Mutation for lysine overproduction in four strains of Saccharomyces cerevisiae" (1978). Undergraduate Theses. 10694.
https://www.ukdr.uplb.edu.ph/etd-undergrad/10694
Document Type
Thesis