Chromosomal comparison of green leafhopper, Nephotettix virescens (Distant), populations sampled from tungro infested and non-tungro infested rice cultivar, Taichung Native 1.

Date

6-1996

Degree

Bachelor of Science in Biology

Adviser/Committee Chair

Adelina A. Barrion

Abstract

Karyological study was conducted to determine the chromo-somal differences between male- green leafhopper populations sampled from tungro and non-tungro infested rice cultivar, Taichung Native I (TN1). Squash preparations of the testes from N. virescens fixed in Farmer's Solution, preserved in 707 ethanol and stained with lacto-aceto-orcein revealed the meiotic cells and chromosomes. Both populations exhibited active meiosis, however, the meiotic indices of the two populations were signifi-cantly different. N. virescens population coming from tungro infested TN1 had a higher meiotic index (86.97%) than those coming from non-tungro infested TN1 (82.13%). At pachytene, chromosome number was confirmed to be 2n=15. Chromosomes of the two populations were significantly different particularly the relative lengths of autosomes and the sex chromosome. Relative lengths of the diakinetic chromosomes of the two populations were significantly different for autosomes 1,2,4,5,6, and 7 at 57. alpha and the sex chromosome at 10% alpha. Results show that N. virescens sampled from tungro infested TN1 were different from those sampled from non-tungro infested TN1 based on their meiotic indices and relative lengths of chromosomes at pachytene and diakinesis.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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