Detection of Xanthomonas oryzae pv. oryzicola, the bacterial leaf streak pathogen on rice, through polymerase chain reaction (PCR) and studies on its genomic DNA.

Date

6-1994

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Asuncion K. Raymundo

Abstract

Sixteen different bacterial isolates were obtained -from various rice plant varieties infected by the bacterial leaf streak pathogen. Further verification of the identity of the organism on the molecular level was necessary. The genomic DNA of each isolate was extracted using the conventional CTAB (cetlytrimethyl ammonium bromide) method by Wilson and IRR1's modified protocol. IRRI's method was simpler, faster, more convenient, and obtained purer DNA extracts. The DNA extracts were digested with Pat 1 to be used for further study on the genetic diversity and population structure of the organism based on RFLP (restriction fragment length polymorphism) analysis. DNA fragments from all 16 strains were amplified by the polymerase chain reaction (PCR) method using oligonucleotide primers Ca and P2, putatively specific for X. o. pv. oryzicola. Thus, the isolates were identified as X. o. pv. oryzicola. Results of the pathogenicity test corroborated with that of the PCR. Upon inoculation on 1R50, the bacterial strains caused the bacterial leaf streak disease. Variation in virulence of the isolates was also observed. Thus, PCR is a simple, rapid, specific and sensitive method which can be successfully used for the identification of specific organisms.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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