Electrophoretic analysis of protein polymorphism in Philippine mallard duck (Anas platyrhynchos Linn.) sampled from Caluan, Laguna

Date

3-2005

Degree

Bachelor of Science in Biology

Major Course

Major in Ecology Genetics

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Adelina A. Barrion

Abstract

The intraspecific variation, through protein polymorphism, of the 80-head population of mallard ducks (Areas platyrhynchos Linn.) from Calauan, Laguna was determined and characterized using starch-gel electrophoresis. From the total sample, consisting of 50 female and 30 male ducks, blood plasma was extracted to analyze three (3) enzymes; namely: acid phosphatase (ACPH), alkaline phosphatase (ALPH), and esterase (EST); and two (2) blood proteins; namely. transferrin (Trf) and albumin (ALB). All protein systems showed more than one (1) locus. ACPH had three (3) isoloci designated as ACPH-1, ACPH-2, and ACPH-3. Likewise, ALPH had three (3) isoloci denoted as ALPH-1, ALPH-2, and ALPH-3. On the other hand, EST exhibited four (4) isoloci noted as EST-1, EST-2, EST-3, and EST-4. Similarly, four (4) isoloci were observed in Trf and designated as Trf-1, Trf-2, Trf-3, and Trf-4. Only two (2) isoloci were detected in ALB — ALB-1 and ALB-2. For the male ducks, all the ALPH isoloci were monomorphic, as well as the ALB isoloci, and the Trf-4 isolocus was not found in the male ducks; the rest of the isoloci were polymorphic. For the female ducks, EST-3, Trf-4, and ALB-2 were monomorphic; the rest were polymorphic. A total of 15 isoloci were observed in the male ducks and 16 isoloci in the female ducks. The genotypes (S/S, S/F, and F/F) of the protein systems were found to be controlled by two (2) codominant alleles, S (slow) and F (fast). However, a third allele, M (medium), was observed only in the genotypes (S/M, M/F, and M/M) of EST-2 locus. The computed value of the degree of polymorphism in the female ducks (0.813) was greater than that of the male ducks (0.600). In the same manner, the average number of alleles in the female ducks (1.875) was greater than the male ducks (1.667). Moreover, the mean heterozygosity of the female ducks (0.342) exceeded that of the male ducks (0.236). The degree of polymorphism and mean heterozygosity in the combined observations were 0.875 and 0.357, respectively.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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