The effects of cadmium and lead on the morphology, growth and membrane lipid peroxidation of mani-mani (Arachis pintoi Krap. & Greg.).

Date

4-2005

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Nina M. Cadiz

Abstract

To determine the effects of cadmium (Cd) and lead (Pb) on the morphology, growth and membrane lipid peroxidation of mani-mani (Arachis pinto! Krap. & Greg.), two experiments were set up. The first set-up consisted of Cd at five level concentrations (5, 10, 15, 20, and 25 uM), while the second setup was Pb at four level concentrations (10, 25, 50, and 100 uM). A more intense browning of leaves was observed in Cd-treated plants compared with Pb. A decrease in root and shoot dry weight was observed with increasing concentration of the metals. The highest concentration of Cd (25 uM) had root and shoot dry weights of 0.1667 and 0.5667 g, respectively. However, no significant difference was observed in root and shoot dry weights of plants treated with Pb. No significant difference was also noted in root length for both metal treatments. In addition, only Cd treated plants showed a significant decrease in the number of nodules. The effect of both metals on nodule diameter, however, was not significant. Tissue analysis showed greater metal concentration in shoots than in roots. Cd treatment increased metal uptake in roots up to 20 uM (3.677 g/kg) but declined at 25 uM (2.745 g/kg). In shoots, however, metal uptake increased up to 15 uM (6.650 g/kg) but declined at 20 uM (6.165 g/kg). On the other hand, Pb uptake of roots and shoots increased significantly with increasing concentration. Root electrolyte leakage increased up to 20 and 50 uM Cd and Pb, respectively but declined at 25 uM (0.8217 mS/cm2) and 100 uM (0.4440 mS/cm2) for Cd and Pb, respectively. Based on the results, mani-mani showed potential as phytoremediation species for sites contaminated with up to 100 uM Pb since Cd showed to be more toxic to this plant than Pb.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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