Genetic diversity in Korean germplasm of rice (Oryza sativa L.) with different levels of resistance to blast in the Philippines.

Date

6-2006

Degree

Bachelor of Science in Biology

Major Course

Major in Ecology Genetics

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ivan Marcelo A. Duka

Abstract

Ninety-six varieties of rice (Oryza saliva L.) from Korea with different levels of resistance to rice blast were grown at the IRRI greenhouse. At 14 days of growth, leaves from each variety were collected and their DNA extracted. In the polymerase chain reaction (PCR), 63 rice microsattelite markers (SSR) were used and the products were detected with 2% agarose gel electrophoresis. Polymorphisms generated by the primers were scored and clustered using NTSYS. Out of the 63 primers, only six were non polymorphic. Polymorphic bands generated by the primers numbered from one to five in each genotype. The degree of polymorphism of each primer was quantified using the polymorphism information content (PIC). The highest PIC value came from RM 8049 (77%), making it the most polymorphic primer. Non polymorphic primers produced the lowest PIC value of 0%. Genetic distance values among the varieties were determined using the genetic distance matrix. Values close to 1 signify great difference between the varieties and values close to 0 signify great similarity. The overall genetic diversity of the 96 Korean rice germplasm was very low (genetic distance value of 0 29 or 29%). Phenotypic and genotypic data were compared and percent similarities between the two were established. Results showed that phenotypic and genotypic data were not completely consistent. Differences between the two may be attributed to other factors such as effect of the environment.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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