Genetic variation patterns in Oryza species with BB and CC genomes revealed using microsatellite markers.

Date

3-2011

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Celia B. Dela Viña

Co-adviser

Kenneth L. Mcnally

Abstract

Studies concerning genetic variation are important in the conservation of wild rice species. This is because wild rice species have useful genes that can be tapped in rice improvement programs. A total of 19 microsatellite markers were used to evaluate the genetic variation of the wild Oryza species with BB and CC genomes. Fifty accessions of 0. punctata (BB), 0. eichingeri (CC) and 0. rhizomatis (CC) were included in this study. All markers were able to detect polymorphisms in the species. A total of 142 alleles were detected across the three species. 0. eichingeri showed the highest genetic variation with gene diversity and heterozygosity values of 0.5856 and 0.1853, respectively in contrast to 0.5309 and 2061 in 0. punctata and 0.5049 and 0.1196 in 0. rhizomatis. Distance and model-based clustering using DarWin and STRUCTURE software, respectively grouped 0. punctata and 0. rhizomatis into distinct clusters. 0. eichingeri accessions were grouped into two clusters according to their geographical location in Sri Lanka and Africa. Results from the model based method also showed 0. rhizomatis accessions having putative introgressions from 0. eichingeri. Results of this study showed that the microsatellite markers originally designed for 0. saliva can be used for microsatellite analysis of the wild Oryza species. Also, there is a moderate level of gene diversity in the Oryza species with BB and CC genomes.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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