Genetic characterization of different Garcinia species through isozyme analysis.

Date

4-2005

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

Isozyme analyses using starch gel electrophoresis on five Garcinia species (G. mangostana, G. binucao, G. kydia, G. lateriflora, and G. linctoria) were conducted to determine genetic variation and identify possible isozyme marker. Enzyme systems used include acid phosphatase (ACP), alcohol dehydrogenase (ADH), alkaline phosphatase (ALP), esterase (EST). glucose-6-phosphate dehydrogenase (G6PD), a-glycerophosphate dehydrogenase (GDH), glutamate oxaloacetate transferase (GOT), isocitrate dehydrogenase (IDH), malic enzyme (MAL), malate dehydrogenase (MDH), phosphoglucoisomerase (PG1), phosphoglucomutase (PGM), and shikimate dehydrogenase (SDH). Two loci were observed for ACP (ACP-1 and ACP-2). ADH (AD1-1-1 and ADH-2), ALP (ALP-1 and ALP-2), EST (EST-1 and EST-2), G6PD (G6PD- I and G6PD-2), GDH (GDH-1 and GDH-2), 1D1-1 (IDH-1 and IDH-2), MDH (MDH-1 and MDH-2), PGI (PGI-1 and PGI-2), PGM (PGM-1 and PGM-2), and SDH (SDH-1 AND SDH-2), while three loci were noted in GOT (GOT-1, GOT-2, and GOT-3) and MAL (MAL-1, MAL-2, and MAL-3). Banding patients of ALP, GDH, 1DH, MAL, and SDH were shared by G. mangostana and G. linctoria. G. binucao, G. kydia, and G. lateriflora had similar banding pattern for G6PD, GDH, 1D1-1, PGI, and PGM. G. mangostana and G. kydia shared a common banding pattern for ACP. G. kydia and alateriflora had similar banding pattern for ADH, and AD11-2 was not detected in G. binucao. The absence of both ALP loci was observed in G. kydia. Two loci for EST were found to be common in the five species, except for G. binucao having no EST-1 locus. G6PD-1 was distinct to G. mangostana, while G. binucao, G. kydia and G. lateriflora shared the same banding pattern for this enzyme. GDH-1 was common to G. mangostana and G. linctoria only. G. kydia and G. lateriflora shared a common banding pattern for GOT, and GOT-3 locus was shared be G. mangostana and G. linctoria only. IDH-1 was common to G. mangostana and G. linctoria. Similar banding pattern of G. binucao, G. kydia, and G. lateriflora for this enzyme was also observed. Four banding patterns for MDH were generated, with G. binucao and G. kydia having similar banding pattern. MAL-3 was distinct to G. kydia and G. lateriflora, while G. binucao had MAL-2 locus only. PGI-1 was common to the five species and PGI-2 distinct to G. linctoria only. 1'GM-2 was distinct to G. linctoria and PGM- I was shared by the five species. SDH-1 was common to the rive Garcinia species, and SDH-2 was distinct to G. kydia and G. lateriflora only. A phylogenetic tree was generated based on similarity index of the five species. G. mangostana and G. tinctoria formed a cluster while G. binucao, G. kydia and G. lateriflora formed another cluster, with a subcluster composed of G. kydia and G. lateriflora.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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