"Antioxidant activities of the chemical constituents of Chinese cedar (Cedrela sinensis A. Juss), Rosemary (Rosmarinus officinalis L.), and Pengu Senna (Cassia sophora L. var. Penghuana)."

Date

4-2004

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn Mae T. Mendoza

Abstract

This study was conducted to evaluate the antioxidant activities of the phytochemical constituents of Chinese cedar (('edrela sinensis A. Juss), rosemary (Rosniarinus qfficinalis L) and Penghu senna (Cassia sophora L var Penghuana) Methanolic extracts of leaves of Chinese cedar, leaves of Penghu senna and leaves and stems of rosemary were fractionated using reverse phase high performance liquid chromatography. Antioxidant activity of the fractions was then determined. Among the three samples, the Chinese cedar fractions showed the highest antioxidant activity, followed by rosemary and then by Penghu senna. Determination of the antioxidant activities of the fractions showed that flavonoids, in general, contributed the most to the total antioxidant activity of the fractions. Carotenoids, and chlorophylls and their derivatives also exhibited antioxidant capacities, although to a lesser extent. Comparison of the antioxidant activities of the fractions to the total antioxidant activity of the crude extract showed that the antioxidant activities of the fractions has non-additive effects, an indication that antagonistic or competitive interactions between physochemicals might occur. Also, certain peaks in the vegetable samples were observed to elute at very similar retention times. Given the same chromatographic conditions, it is possible that the three vegetables share similar antioxidants.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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