Enhancement of transplanting survival of embryo-cultured macapuno (Cocos nucifera Linn.) seedlings by pre- treatment with bacteria.
Date
3-1985
Degree
Bachelor of Science in Biology
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Ruben B. Aspiras
Abstract
This study was designed to determine tie effectiveness of utilizing root-colonizing bacteria in protecting roots of embryo-cultured macapuno seedlings from harm by disease. causing microorganisms, to treat roots of these seedlings with Bacillus polymyxa and Azospirillum lipoferum, to in-crease seedling survival after transplanting and to deter-mine if these bacteria would continue to be active in nitrogen-fixation in association with roots of macapuno seedlings. Embryo-cultured seedlings of macapuno (Cocos nucifera Linn.) approximately six months old were subjected to thirteen different treatments, each treatment replicated eight times. The treatments comprised of two inoculations, i.e., B. polymyxa, A. lipoferum and the uninoculated control. With these, the plants were planted in either sterile or non-sterile soil, with or without actidione. The usual practice of rearing the macapuno seedlings in the Macapuno Lab. was included. The number of living seedlings after two months was recorded. Roots were obtained from the surviving seedlings and the microorganisms were subcultured in the N-deficient semi-solid glucose yeast extract agar for nitrogenase assay using the acetylene reduction technique. Results show that bacterial treatment as well as actidione treatment were not different in enhancing transplanting survival. Sterilization treatment showed a difference in enhancement. Positive nitrogenase activity indicates that the bacteria were still present in the roots six months after bacterial inoculation. B. polymyxa had a nitrogenase activity that is higher than that of A. lopoferum. Microscopic revealed that the bacteria inoculated were still present in the treated seedlings. In view of this, enhancement of transplanting survival shows with the use of more refined methods.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
Thesis
Recommended Citation
Balaaldia, Catherine B., "Enhancement of transplanting survival of embryo-cultured macapuno (Cocos nucifera Linn.) seedlings by pre- treatment with bacteria." (1985). Undergraduate Theses. 10789.
https://www.ukdr.uplb.edu.ph/etd-undergrad/10789
Document Type
Thesis
