Comparative cytogenetic analysis of Oriental migratory locust (Locusta migratoria manilensis Meyen) populations from Tarlac and Pampanga.

Date

4-1996

Degree

Bachelor of Science in Biology

Major Course

Major in Ecology Genetics

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Abstract

Natural populations of locust from Tarlac and Pampanga were collected and fixed in 3:1 95% ethanol and glacial acetic acid. Testes was dissected and slides were prepared using squash technique. For both populations, chromosome number of 2n = 25 (12 II + X0) was consistently observed at diakinesis. The average computed meiotic index from Tarlac and Pampanga was 0.73 and 0.68 respectively. Normal chromosome behavior were observed from both populations at the different substages of prophase I with 12 bivalents and an X chromosome which is a univalent. Based on pachytene analysis the relative mean length of chromosomes from Tarlac population ranges from 1.0 to 0.17 while in Pampanga population it was equal to 1.00 to 0.13. Based on statistical analysis, there are no significant differences on the relative mean length of the chromosome number 1, 2, 10, 11 and 12. The rest of the chromosomes vary significantly in terms of their relative mean length. For both populations, the abnormalities observed were the occurrence of one laggard per cell at metaphase I. This was also observed at anaphase I as well as bridge formation. In spite of the occurrence of these abnormalities, cells at telophase I were still normal indicating that the laggards were able to catch up with the other chromosomes and were included in the daughter cells. Hence, it is likely that an equal distribution of chromosomes to daughter cells occurred. Cell at meiosis II for both populations were also normal. Based on the statistical analysis, there are no significant differences in terms of the meiotic index and chromosome behavior of the cells from the two populations. Thus , it can be concluded that the two populations were not diverse species.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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