DNA Fingerprinting of Ralstonia solanacearum (E.F. smith) Yabuuchi et al., isolates from soil collected from potato (Solanum tuberosum) growing areas in Bukidnon, Philippines

Date

12-2011

Degree

Bachelor of Science in Agriculture

Major Course

Major in Plant Pathology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Ardales, Edna V.

Abstract

Fifty-two Ralstonia solanacearum isolates were collected from six sites in Bukidnon province and were characterized based on colony morphology on TZCA, pathogenicity to tomato, phylotyping and DNA fingerprinting by Rep-PCR using the repetitive elements REP and BOX as primers. Phylotype-specific multiplex PCR confirmed that all the isolates are R. solanacearum and belonged to phylotype I. All isolates were able to infect tomato but varied in the degree of infection caused and the time required for initiation of wilting. Molecular characterization by Rep-PCR identified 18 REP and 21 BOX haplotypes from the 52 isolates. Cluster analysis by UPGMA grouped the REP and BOX haplotypes as well as the composite haplotypes, generated by combining the REP and BOX binary data, into 8, 10, and 12 clusters, respectively at 70%. Majority of the clusters formed contained isolates collected from the same site suggesting the isolates grouped according to geographical origin. Out-grouping of the isolates was also evident. The haplotypic diversity estimates of the R. solanacearum collection were calculated using Nei’s haplotypic diversity index. High total haplotypic diversity estimates of 0.93 and 0.96 were obtained based on REP and BOX, respectively.

Language

English

Location

UPLB Main Library Special Collections Section

Call Number

Thesis

Document Type

Thesis

This document is currently not available here.

Share

COinS