Genetic diversity study analysis using SSR markers of varieties of rice (Oryza sativa L.) from Indonesia and Philippines (Arakan, Cotabato) and relation to resistance rice blast pathogen (Magnaporthe grisea) races found in the Philippines

Date

4-2007

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Carmina C. Manuel

Co-adviser

Casiana M. Vera Cruz

Abstract

Genetic diversity of the traditional and improved traditional varieties of rice (Oryza saliva L.) from Indonesia, as well as, varieties from Arakan, Cotabato, Philippines were screened using Simple Sequence Repeat (SSR) markers. A total of 30 SSR markers were used for the Indonesian rice varieties, and 33 markers were used for the Arakan rice varieties. The polymorphic information content (PIC) value of the markers ranged from 0 to 0.855 for Indonesian varieties. while the PIC value range in the Arakan varieties was from 0 (RM504) to 0.814 (RM507). A phenotypic tree plot was constructed based on diseased leaf area response (DLA) to nine rice blast races in the Philippines using the Numerical Taxonomy System (NTSYS) software. The phenotypic similarity within Indonesian varieties ranges from 0.08 to 1. while the Arakan estimate for phenotypic similarity ranged from 0.09 to 0.89. Based on the genetic tree plot constructed, the estimate for genetic similarity ranged from 0.24 to 0.91 (between Sirendah 7A and Sirendah 7B) and 0.21 to 0.94 (between Dinorado 35 and Dinorado 36) for Indonesian and Arakan varieties, respectively. These figures suggest that both germplasm are phenotypically and genetically diverse. Using the Statistical Analysis System (SAS) software. the stepwise regression analysis between the SSR markers and phenotypic response for each rice blast race was calculated. The data showed that 59 alleles in the Indonesian rice germplasm, 91 alleles in the Arakan rice varieties amplified by the SSR markers were significantly correlated to rice blast resistance at 5% level of significance.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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