In vivo and in vitro studies on the cytogenetic effect of warfarin on Rattus rattus mindanensis.

Date

10-1982

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Abstract

To determine the cytogenetic effect of warfarin on Rattus rattus mindanensis, in vivo and in vitro studies were conducted. For in vivo study,12 rats were subjected to 0 mg/kg, 2.5 mg/kg, 5.0 mg/kg and 10 mg/kg warfarin in corn oil injected intraperitoneally for five consecutive days. Results showed that warfarin failed to inhibit the mitotic and meiotic activity but significantly induced chromosonal aberrations including multivalent/quadrivalent formation at diakinesis. For the in vivo study, peripheral blood cells were cultured for three days and each culture received various doses of warfarin: 0.0%, 0.0025% and 0.01;1. Unsuccessful growth was obtained from culture of normal and treated leucocytes with mitotic activity ranging from 0.00— 2.25%. With this, in vivo study was not adequately supported by in vitro data. However, the in vivo findings suggest that warfarin can be considered as a chemical for genetic control They have been postulated to intercalate in stacked bases of DNA. Recommendations for in vitro study were included in the discussion.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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