Genetic variability in Oryza officinalis wall ex watt detected using microsatellite markers.

Date

4-2011

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Celia B. Dela Viña

Abstract

Genetic variation in 72 accessions of the wild rice CC genome species, Oryza ojficinalis Wall ex Watt was analyzed using 19 microsatellite markers. A total number of 97 alleles were detected from 16 polymorphic markers. The most informative marker (RM10) showed a PIC = 0.7524. RM332 was also considered an informative marker (PlC 0.5121) because of high levels of gene diversity (0.5956) and heterozygosity (0.9859). Gene diversity and heterozygosity ranged from 0.0941 to 0.7822 and 0 to 0.9859, respectively. High diversity indices were observed in populations from India, Indonesia, Malaysia, and Myanmar. Cluster analysis by distance-based and model-based methods differentiated the populations according to their geographic distribution. Populations originating in South (India) and East (China) Asia formed a group distinct from populations found in Southeast Asia. The results of this study can help to improve the conservation of O. officinalis and aid in the choice of germplasm for use in breeding programs.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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