Isozyme polymorphism of oriental fruit fly Bactrocera occipitalis Drew and Hancock and B. philippinensis (Bezzi) (Diptera: Tephritidae) from Puerto Princesa, Palawan

Date

4-2009

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

Isozyme analysis was used to determine and compare the extent of genetic variability within and between the two species of fruit fly Bactrocera occipitalis and B. philippinensis from Puerto Princesa, Palawan. Six presumptive loci coding for four enzymes were revealed. Acid phosphatase (ACP-1) and malic enzyme (ME-1) had one presumptive locus each, while alkaline phosphatase (ALP-1, ALP-2) and esterase (EST-1, EST-2) had two loci each. Two alleles, S and F, were found for ALP-1, ALP-2, EST-1, EST-2 and ME-1. ACP-I showed only S allele. Degree of polymorphism was higher for male B. philippinensis (1.00) compared to male B. occipitalis (0.83). The female B. occipitalis showed higher P value (0.60) compare to B. philippinensis (0.50). Using Numerical Taxonomy and Multivariate Analysis System (NTSYS) the dendrogram constructed for each species showed no major clusters. No clustering was also noted when the data for both species were combined. Based on the four isozymes, B. occipitalis and B. philippinensis were found to be related.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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