Morphology of some Datura species and alkaloid profiles of local representatives Datura mollis and Datura candida Pers.

Date

4-2005

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Lourdes B. Cardenas

Abstract

Morphological characterization was done for four Datum species namely D. Sfra111011iUM, D. alba, D. mills and D. candida.. D. stramonium and D. alba are relatively similar being small plants that bear fruits. On the other hand, D. mollis and D. candida are small trees that do not bear fruits. Test procedures were done to profile alkaloid content of two local Datura species, D. mollis Saff. and D. candida Pers. which are found in the vicinity of UP Los Bafios. The two procedures were: methanol extraction of alkaloids from crude drug with alkaloid content of > I% of dried weight and ether extraction of alkaloids from crude drug that give chlorophyll zones. Another procedure was done to analyze whether beer is good for alkaloid extraction. This was done because teenage victims used beer as a medium. Literature shows that atropine is the most potent alkaloid in Datum. Indeed, atropine was found in both D. monis and D. candida using the two general procedures. The atropine traveled with the expected Rf value of 0.25. The results showed that D. !milts has greater alkaloid content than the D. candida due to more and thicker bands. For both species, flowers have greater alkaloid content than leaves. The result contradicts previous experiment on D. mollis and D. !noel L. where leaves showed greater alkaloid content than flowers. The result on the beer-chloroform extraction was negative. There were no bands observed suggesting that brand beer (San Miguel Light Beer) did not optimize alkaloid extraction. The possible reason why teenagers still experienced hallucinations was the fact that they used the crude extract itself.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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