Cloning and sequence analysis of 3-Ketoacyl acyl carrier protein synthase III (KAS III) gene in coconut (Cocos nucifera L.).

Date

4-2005

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Co-adviser

Marni E. Cueno

Abstract

The putative gene encoding 3- ketoacyl acyl carrier protein synthase III (KAS III) gene, which catalyzes the initial condensation step of acetyl CoA and malonyl CoA in fatty acid biosynthesis, was obtained from the PCR-based 3'-RACE. The putative coconut KAS In gene was then cloned into a TOPO5 vector. Partial DNA. sequence showed that the putative gene has 332 bases from the primer up to the poly-A tail. Open reading frame was set at frame +1 since the primer was found to be intact. The coding region has 31 amino acids encoded by 93 nucleotides. DNA sequence comparison using BLASTN showed hits from ribosomal protein L27 of plants and animals. Conceptual translation of the putative KAS III gene sequence using BLASTX generated 93 out of 101 hits showing KAS Ills from other organisms. Matches include KAS Ills from Spinacia oleracea, Cuphea Glycine max, Elais guineensis, Pisum sativum, Perilla frutescens, and Arabidopsis ihaliana. In bacteria, similarities were found in Bacillus subiilis, B. clausii, Streptococcus thermophilus, Campylobacier jejuni, and Synechocystis sp. Alignments showed 100% homology only to the six amino acids (DITAAC) that are said to be conserved in KAS Ills in the region of the active-site cysteine. This indicated that the cloned gene may possibly be an isoform of KAS III gene in coconut. This study set the groundwork for full-length elucidation of the KAS III gene in coconut.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

This document is currently not available here.

Share

COinS