Hybridity testing of three F1 hybrids of tomato (Lycopersicon esculentum Miller) using simple sequence repeat (SSR) marker.

Date

4-2003

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Neilyn P Ona-Villa

Co-adviser

Conrado H. Balatero

Abstract

Among the four protocols tried for DNA extraction from tomatoes, the protocol of DNA extraction from leaves was identified as the best protocol for DNA extraction from tomatoes in terms of DNA quality and quantity. Three tomato F1 hybrids (F,6137 or "Assunta", F16193, and F161374 or "Ara") developed by the Institute of Plant Breeding, College of Agriculture, University of the Philippines Los Banos were tested for hybridity using SSR analysis. Twenty-seven primers were screened initially for polymorphism and two SSR polymorphic markers were identified: SSR primer 1, which is polymorphic to F16137 and FI6193; and SSR primer 2, which is polymorphic to F16174. These polymorphic markers were used to successfully assess the hybridity of 20 F1 seedlots of each hybrid. SSR primer 1 detected 86 % hybridity in F16137 ("Assunta") and 90 % hybridity in F16193; while SSR primer 2 detected 100 % hybridity in F16174 ("Ara"). The results of the study have demonstrated that SSR can be used as early detection marker for hybridity testing in tomatoes under local laboratory conditions. Compared to traditional field testing procedure such as grow outs, the use of SSR could potentially save time, money, and effort. So far, this is the first report of successful development and utilization of SSR markers for hybridity testing of tomatoes in the Philippines

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

This document is currently not available here.

Share

COinS