"Comparative changes in activity of bacterial polygalacturonase and cellulase in resistant and susceptible tomato (Lycopersicon esculentum Mill.) in response to the bacterial wilt pathogen (Pseudomonas solanacearum E. F. Smith)."

Date

4-1993

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn Mae T. Mendoza

Abstract

Biochemical changes in the activity of polygalacturonase and cellulase in both resistant (Accession # 508) and susceptible (Bacolor) tomato plants were analyzed. There was an increase in the activity of polygalacturonase from the first day until the third, fourth or fifth day of infection. This trend was observed for both the susceptible and resist-ant varieties. The increase in activity were 86.1%, 96.9% and 88.3% for the roots, stems and leaves of the susceptible plant, respectively. For the resistant plant, the increase in activity were 89.2%, 94.3% and 90.8% for the roots, stems and leaves, respectively. A considerable decrease in activity was observed during the advanced stage of the disease. The decrease in the activity in the the susceptible plant were 73.2% for the roots, 89.5% for the stems' and 85.2% for the leaves. The decrease in the activity of the resistant plant were 91.6%, 77.2% and 83.3% for the roots, stems and leaves respectively. Enzyme activity was highest in the leaves followed by the stems and then the roots. In the susceptible plant, the activity in the leaf was 19.6% higher than in the stems and is 63.2% higher than in the roots. Activity in the leaf was 26.1% and 57.4% higher than that in the stems and roots, respectively. For cellulase, the activity increased by 72.4% on the later stage of the infection in stems of the susceptible plants while for the resistant plants, the activity increased for only 51.2% on the fourth day and the values seem to fluctuate. For both polygalacturonase and cellulase, enzyme activity was higher in the susceptible plants than in the resist-ant ones. Polygalacturonase activity in the susceptible roots was 9.1 to 78.3% higher than in the resistant roots. For the stems, activity was higher by 15.8 to 88% and for the leaves, the activity in the susceptible plant was 27 to 69.5% greater than the resistant one. Cellolase activity was 25.2 to 76.6% higher in the susceptible stems than in the resistant stems.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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