Cytology of gumamela, Hibiscus rosasinensis L. Var Sinensis [Malvales : Malvaceae].

Date

6-1992

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Adelina A. Barrion

Abstract

Acetocarmine squash preparations of Farmer's fixed anther, of H. rosasinensis showed peculiar cytogenetic behavior. At Diakinesis, 18 combinations of chromosomes were observed. The most dominant combination which occurred in 16% of cells was 23 II or 2n = 46. Pachytene analysis revealed that the relative lengths of the chromosomes ranged from 0.023 to 0.070. The arm ratios ranged from 2.25 to 4.86. Three kinds of chromosomes were determined based on Levan's classification. Chromosome pair 17 was the only metacentric : chromosome pairs 5, 6, 10, 11, 12, 15, 16, 18, 19, 20, 21. 22 and 23 were sub-metacentric and chromosomes 1, 2, 3, 4, 7, 8, 9, 13 and 14 were sub-telocentric . Opt anaphase and telophase, differential disjunctions and asynchronous movements were observed. Many chromosomes appeared to be laggards. Based on varying sizes and shapes of the chromosomes, H. rosasinensis possessed asymmetric karyotype. Also, cytokinesis seemed to be disturbed, ultimately resulting to occurrences of polynuclei and macro-micronuclei. The pollen fertility of H. rosasinensis was 95.9% . Thus, the peculiar cellular and chromosomal behaviors were the species adaptive mechanisms to insure success in nature.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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